Nucleic acid gel stains are inherently dangerous because of their potential to cause genetic mutation. Few would dispute that one of the best ways to reduce or eliminate their toxicity is to prevent the dyes from entering the cells in the first place. But the challenge is: How do you do it without compromising performance? The answer is innovation. GelRed™ and GelGreen™ are patented breakthrough nucleic acid gel stains and the only gel stains that offer the combination of: 1) cell membrane impermeability for true safety;* 2) unrivaled sensitivity; 3) perfect instrument compatibility; 4) superior stability; and 5) compatibility with all downstream manipulations.

Continuing our innovation, now we have made GelRed™ and GelGreen™ even better by introducing yet another breakthrough – GelRed™ and GelGreen™ Prestaining Kits for ultimate safety, performance and value. Each kit contains an optimally formulated 6X GelRed™ or GelGreen™ loading buffer and a superior proprietary running buffer. With these new kits, you enjoy the following additional benefits:

    GelRedTM     GelGreenTM
Figure 1.Prestaining gel electrophoreses of DNA ladders from seven major vendors.
  • Ultimate safety: Absolutely minimal exposure to a dye that already has an excellent safety profile.
  • Extremely simple procedure: Simply load your sample in the GelRed™ or GelGreen™ loading buffer and run your gel in the proprietary running buffer provided in the kit. No additional dye in the gel or running buffer and no post staining is necessary.
  • Compatibility with nucleic acid ladders: Work well with all commercial DNA/RNA ladders. No more migration or band distortion problems.
    Re-usability: Gels may be re-used if there are empty wells.
  • Flexibility for fast electrophoresis: Amenable to high-voltage electrophoresis to shorten running time without overheating.
  • Unmatched value: Enough loading buffer and running buffer are provided for analysis of up to 100 mini gels.

* A complete safety report can be downloaded at Biotium website.
** GelRed, GelGreen and their uses are covered by US patent No. 7,601,498 and other pending US and international patents; GelRed and GelGreen are trademarks of Biotium, Inc.

    SYBR Dyes GelGreen GelRed  
  A) 5 min. incubation The best way to make a DNA-binding dye safe is to prevent it from going into cells in the first place.
  B) 30 min incubation
 
Figure 2. HeLa cells were incubated at 37 oC with 1X of SYBR Green I, SYBR Safe, GelGreen and GelRed, respectively. Images were taken following incubation for 5 min (panel A) and 30 min (panel B), respectively.
SYBR Green I and SYBR Safe entered into cells rapidly as evident from the bright green nuclear staining (only images from SYBR Green are shown). However, GelRed and GelGreen were unable to cross cell membranes as shown by the lack of any fluorescence staining.

 
   
  GelRed and GelGreen Prestaining flyer
  GelRed and GelGreen slide presentation
  GelRed GelGreen Safety Report here
     
     

 
 

Table A.Ordering Information

   Cat#
Product Name
Unit Size
Unit Price
  31010
GelRedTM nucleic acid gel prestaining kit with "200bp" and "1.5Kb" tracking dyes(each kit sufficient for 100 mini gels)
 Each
    $95
  31011
GelRedTMnucleic acid gel prestaining kit with "50bp" tracking dye(each kit sufficient for 100 mini gels)
 Each
    $95
  31012
GelGreenTM nucleic acid gel prestaining kit with "200bp" and "1.5Kb" tracking dyes(each kit sufficient for 100 mini gels)
 Each
    $95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

Table B.Selection Guide for GelRedTM/GelGreenTM Prestaining Kits

If You Have the Following Condition

Select This Kit

Medium to large DNA fragments

UV(300nm) excitation; EtBr filter

Cat# 31010, GelRedTM nucleic acid gel prestaining kit with “200bp” and “1500-2000bp” tracking dyes

UV(254nm), blue light(470 nm) or 488 nm laser excitation; green filter

Cat# 31012, GelGreenTM nucleic acid gel prestaining kit with “200bp” and “1500-2000bp” tracking dyes

Small DNA/RNA fragments; PAGE gels

UV(300 nm) excitation; EtBr filter

Cat# 31011, GelRedTM nucleic acid gel prestaining kit with “50bp” tracking dye

UV(254 nm), blue light(470 nm) or 488 nm laser excitation; green filter

Cat# 31013, GelGreenTM nucleic acid gel prestaining kit with “50bp” tracking dye

 
 

 
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