CF™Dyes  
 

The next-generation dyes for protein labeling
CF dye flyer
Near IR CF dye flyer
 

CF dyes are a series of fluorescent dyes with superior brightness and photostability. Primarily designed for labeling antibodies, many of our CF dyes (near IR CF dyes in particular) outperform the Alexa Fluor dyes (For more detailed comparison, please download CF dye flyer and Near IR CF dye flyer ). CF dye antibody conjugates generally give better signal to noise ratio than Alexa Fluor antibody conjugates because of the fewer charges CF dyes carry.

CF dye products are currently offered in three categories: CF dye-labeled secondary antibodies (Table A); other CF dye-labeled products (Table B); and reactive CF dyes and labeling kits (Table C). More CF dye products will be offered in the near future as they become available. We also do custom conjugations if you have a primary antibody or any particular substrate molecule you would like us to label with one of the CF dyes.

For more information on each product, click the product name in the table. To license CF dye technology, please contact btinfo@biotium.com.

 
   
 
 
Figure 1. HeLa cells were stained with CF 555 phalloidin (red), mouse anti-α-tubulin followed by goat anti-mouse CF 488A IgG (green) and DAPI (blue), respectively.
   
 
 
Figure 2. HeLa cells were stained with mouse anti-golgin 97 and rabbit anti-COX IV primary antibodies followed by CF488A goat anti-mouse and CF555 goat anti-rabbit secondary antibodies. CF647-phalloidin was used to label F-actin and Hoechst 33342 was used to stain for DNA. (Green=golgi, red=mitochondria, blue=nucleus, and magenta=cytoskeleton).
   
 
 
Figure 3. Rat hippocampal neurons stained with SynaptoGreen C4 (also called FM1-43, cat# 70020) and CF647 hydrazide (cat# 70020). SynaptoGreen C4 stains synaptic vesicles (green) while CF647 hydrazide (cat# 92136) stains the cell bodies (red). (Contributed by Hang Zhou from Professor Guosong Liu's lab in Tsinghua University, China)
 
Fluorescence Emission Spectra of CF Dyes
 

 

Table A. CF Dye-labeled Secondary Antibody Conjugates

Cat#
Product Name
Abs/Em* (nm)
Unit Size
Unit Price
20080
CF405S Goat Anti-Mouse IgG (H+L), 2 mg/mL
0.5 mL
$130
20082
CF405S Goat Anti-Rabbit IgG (H+L), 2 mg/mL
0.5 mL
$130
20016
CF488A Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL
0.5 mL
$150
20014
CF488A Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit and Sheep), 2 mg/mL
0.5 mL
$150
20015
CF488A Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL
0.5 mL
$150
20011
CF488A F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
490/515
0.25 mL
$105
20013
CF488A F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
490/515
0.25 mL
$105
20017
CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL
0.5 mL
$150
20010
CF488A Goat Anti-Mouse IgG (H+L), 2 mg/mL
490/515
0.5 mL
$130
20012
CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL
490/515
0.5 mL
$130
20039
CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL
0.5 mL
$150
20037
CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL
0.5 mL
$150
20038
CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL
0.5 mL
$150
20032
CF555 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
555/565
0.25 mL
$110.25
20035
CF555 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
555/565
0.25 mL
$110.25
20036
CF555 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL
555/565
0.5 mL
$130
20030
CF555 Goat Anti-Mouse IgG (H+L), 2 mg/mL
555/565
0.5 mL
$136.5
20033
CF555 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
555/565
0.5 mL
$136.5
00009
CF633 a-Bungarotoxin
0.5 mg
$300
20127
CF633 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL
0.5 mL
$150
20124
CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL
0.5 mL
$150
20125
CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL
0.5 mL
$150
20137
CF633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL
0.5 mL
$150
20134
CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL
0.5 mL
$150
20126
CF633 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20129
CF633 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL
0.5 mL
$130
20132
CF633 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20120
CF633 Goat anti-mouse IgG (H+L), 2 mg/mL
0.5 mL
$130
20130
CF633 Goat anti-mouse IgG (H+L), F(ab’)2 fragment, 2 mg/mL
0.25 mL
$105
20121
CF633 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20122
CF633 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
0.5 mL
$130
20131
CF633 Goat Anti-Rabbit IgG (H+L), F(ab’)2 fragment, 2 mg/mL
0.25 mL
$105
20123
CF633 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20133
CF633 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20138
CF633 Goat Anti-Swine IgG (H+L), 2 mg/mL
0.5 mL
$130
92156
CF633 hydrazide
1 mg
$150
92026
CF633 maleimide
1 umole
$195
00046
CF633 Phalloidin
300 U
$290
20128
CF633 Rabbit Anti-Goat IgG (H+L), 2 mg/mL
0.5 mL
$130
20136
CF633 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
20135
CF633 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL
0.5 mL
$150
29037
CF633 Streptavidin
1 mg
$160
92133
CF633 succinimidyl ester
1 umole
$210
92053
CF633, aminooxy
1 mg
$390
20048
CF647 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL
0.5 mL
$150
20046
CF647 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL
0.5 mL
$150
20047
CF647 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL
0.5 mL
$150
20042
CF647 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL
650/665
0.25 mL
$110.25
20045
CF647 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL
650/665
0.25 mL
$110.25
20040
CF647 Goat Anti-Mouse IgG (H+L), 2 mg/mL
650/665
0.5 mL
$136.5
20043
CF647 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
650/665
0.5 mL
$136.5
20050
CF660 Goat Anti-Mouse IgG (H+L), 2 mg/mL
667/685
0.5 mL
$130
20053
CF660 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
667/685
0.5 mL
$130
20065
CF680 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL
681/698
0.25 mL
$160
20067
CF680 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL
681/698
0.25 mL
$160
20070
CF750 Goat Anti-Mouse IgG (H+L), 2 mg/mL
755/777
0.5 mL
$150
20073
CF750 Goat Anti-Rabbit IgG (H+L), 2 mg/mL
755/777
0.5 mL
$150
20077
CF770 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL
770/797
0.25 mL
$160
20078
CF770 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL
770/797
0.25 mL
$160

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

Table B. Other CF dye-labeled Products

Cat#
Product Name
Abs/Em* (nm)
Application
Unit Size
Unit Price
29005
 Annexin V, CF488A conjugate, 50 ug/mL
490/515
Apoptosis
0.5 mL
$220
29004
 Annexin V, CF555 conjugate, 50 ug/mL
555/565
Apoptosis
0.5 mL
$220
29003
 Annexin V, CF647 conjugate, 50ug/mL
650/665
Apoptosis
0.5 mL
$220
29008
 CF633 Annexin V, 50 ug/mL
0.5 mL
$220
00034
 Phalloidin, CF405 conjugate
405/460
Cell skeleton (actin)
300U
$290
00042
 Phalloidin, CF488A conjugate
488/516
Cell skeleton (actin)
300 U
$290
00040
 Phalloidin, CF555 conjugate
555/565
Cell skeleton (actin)
300 U
$290
00041
 Phalloidin, CF647 conjugate
650/665
Cell skeleton (actin)
300 U
$290

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

Table C. Reactive CF Dyes and Labeling Kits

Cat#
Product Name
Abs/Em* (nm)
Application
Unit Size
Unit Price
92120
 CF488A NHS ester
488/516
Amine-reactive
1 umole
$210
92130
 CF555 NHS ester
552/574
Amine-reactive
1 umole
$210
92237
 CF633 SE microscale protein labeling kit
3 labelings
$360
92217
 CF633 SE protein labeling kit
3 labelings
$350
92135
 CF647 NHS ester
650/674
Amine-reactive
1 umole
$210
92137
 CF660 NHS ester
664/685
Amine-reactive
1 umole
$210
92139
 CF680 NHS ester
679/701
Amine-reactive
1 umole
$225
92142
 CF750 NHS ester
752/786
Amine-reactive
1 umole
$225
92150
 CF770 NHS ester
766/800
Amine-reactive
1 umole
$225
92160
 VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF680 SE
679/701
Amine-reactive
3 labelings
$395
92161
 VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF750 SE
752/786
Amine-reactive
3 labelings
$395
92162
 VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF770 SE
766/800
Amine-reactive
3 labelings
$395

*Absorption/emission wavelengths for free CF dyes and CF dyes conjugated to proteins are slightly different. See Table A

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

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