Applications > Cell Biology > Antibodies, strepavidin and lectins

Mix-n-Stain™ CF™ Dye Antibody Labeling Kits

Covalently label 5 to 100 ug of an Antibody with the Brightest Dyes in 30 min — It’s a Revolution!

Mix-n-Stain™Antibody Labeling Kits flyer

Mix-n-Stain™ CF™ dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF™ dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

Another major advantage of the labeling kits is our superior CF™ dyes, many of which are brighter and more photostable than Alexa Fluor® dyes and other similar commercial dyes. With a large list of CF™ dyes of wavelengths covering the entire visible and near-infrared spectra, you have unprecedented freedom to label any commercial primary antibody with a color of your choice, making multi-color detection simple and affordable.

Features

<30 s hands-on time plus 30 min waiting time
No need to purify
Tolerate common antibody stabilizers
Selection of the brightest dyes with wavelengths covering the entire visible and near-IR spectra
Covalently label any amount of an antibody from 5 ug to 100 ug by selecting an appropriate kit

Frequently Asked Questions and Answers:

Q: Is the labeling chemical or physical?
A: The labeling is chemical, which means the dye is covalently attached to the antibody. Covalent labeling ensures no dye transfer or diffusion in multi-color detection employing multiple antibodies labeled with different CF Mix-n-Stain kits.

Figure . HeLa cells were stained with b-tubulin IgM conjugated with CF™633 Mix-n-Stain (red), followed by CF™488A phalloidin (green) and DAPI (blue). Images were captured on a Zeiss 510 Meta Confocal microscope.

Q: What are the advantages of Mix-n-Stain kits over Invitrogen’s Zenon antibody labeling kits?
A: There are four major advantages: 1) the CF dye is covalently attached to the antibody to eliminate dye transfer or diffusion between antibodies during multi-color staining; 2) Mix-n-Stain conjugates are stable for several months in storage buffer whereas Zenon labeling reagents are required to be used within 30 minutes; 3) Mix-n-Stain conjugates are less bulky because the dyes are directly linked to the antibody, unlike Zenon conjugates which use antibody fragments or other linkers; 4) No dye/protein optimization is needed, just mix and then stain. More >>

Table A. Mix-n-Stain™ product list

Cat#	Product Name	Unit Size	Unit Price	Dye Flyer	Protocol	Mix-n-Stain Flyer
92266	Mix-n-Stain Biotin antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92244	Mix-n-Stain Biotin antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92286	Mix-n-Stain Biotin antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92250	Mix-n-Stain CF350 antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92230	Mix-n-Stain CF350 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92270	Mix-n-Stain CF350 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92252	Mix-n-Stain CF405M Antibody Labeling Kit, 1X(20-50ug) labeling	1 labeling	$105
92232	Mix-n-Stain CF405M Antibody Labeling Kit, 1X(50-100ug) labeling	1 labeling	$115
92272	Mix-n-Stain CF405M antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92251	Mix-n-Stain CF405S antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92231	Mix-n-Stain CF405S antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92271	Mix-n-Stain CF405S antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92253	Mix-n-Stain CF488A antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92233	Mix-n-Stain CF488A antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92273	Mix-n-Stain CF488A antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92267	Mix-n-Stain CF543 antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92247	Mix-n-Stain CF543 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92287	Mix-n-Stain CF543 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92254	Mix-n-Stain CF555 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92234	Mix-n-Stain CF555 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92274	Mix-n-Stain CF555 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92255	Mix-n-Stain CF568 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92235	Mix-n-Stain CF568 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92275	Mix-n-Stain CF568 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92256	Mix-n-Stain CF594 antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92236	Mix-n-Stain CF594 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92276	Mix-n-Stain CF594 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92257	Mix-n-Stain CF633 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92237	Mix-n-Stain CF633 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92277	Mix-n-Stain CF633 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92258	Mix-n-Stain CF640R antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92245	Mix-n-Stain CF640R antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92278	Mix-n-Stain CF640R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92259	Mix-n-Stain CF647 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92238	Mix-n-Stain CF647 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92279	Mix-n-Stain CF647 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92260	Mix-n-Stain CF660C antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92239	Mix-n-Stain CF660C antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92280	Mix-n-Stain CF660C antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92261	Mix-n-Stain CF660R antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92243	Mix-n-Stain CF660R antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92281	Mix-n-Stain CF660R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92262	Mix-n-Stain CF680 antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92240	Mix-n-Stain CF680 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92282	Mix-n-Stain CF680 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92263	Mix-n-Stain CF680R antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92246	Mix-n-Stain CF680R antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92283	Mix-n-Stain CF680R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92264	Mix-n-Stain CF750 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92241	Mix-n-Stain CF750 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92284	Mix-n-Stain CF750 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92265	Mix-n-Stain CF770 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92242	Mix-n-Stain CF770 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92285	Mix-n-Stain CF770 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92268	Mix-n-Stain CF790 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92248	Mix-n-Stain CF790 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92288	Mix-n-Stain CF790 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.