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Applications > Cell Biology > Antibodies, strepavidin and lectins

CF™640R Dye

A highly photostable far-red dye for the 640 nm diode laser

  CF640R dye flyer
  CF dye selection guide

CF™640R is a rhodamine-based far-red fluorescent dye with excitation and emission maxima very similar to those of Cy™5 and Alexa Fluor® 647 (Figure 1). CF™640R is much brighter than Cy™5 and at least as bright as Alexa Fluor® 647 (Figure 2). A major advantage of CF™640R over Cy™ 5 and Alexa Fluor® 647 is its exceptional photostability. Cy™5 and Alexa Fluor® 647 are cyanine-based dyes and, like other cyanine dyes in general, have intrinsically poor photostability. CF™640R is also superior to Atto™ 647N, another spectrally similar dye frequently used in single-molecule imaging. As shown in Figures 2 and 3, CF™640R is significantly brighter and more photostable than the Atto™ dye. The combination of excellent brightness and photostability makes CF™640R ideal for confocal microscopy, single- molecule imaging and other demanding applications based on fluorescence detection.
A list of CF™640R-based products are shown in Table 1. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary

  • Abs/Em Maxima: 642/662 nm
  • Extinction coefficient: 105,000
  • Molecular weight: 832
  • Flow cytometry laser line: 633, 635 or 640 nm
  • Microscopy laser line: 633, 635 or 640 nm
  • Direct replacement for: Cy™5, Alexa Fluor® 647 and ATTO™ 647N

Advantages

  • Has the best photostability among dyes with Cy™5-like spectra
  • Yields highly fluorescent protein conjugates
  • Very water-soluble and pH-insensitive
  
    Figure 1. Absorption and emission spectra of CF™640R and Cy™5 conjugated to goat anti-mouse IgG in PBS.
 
Figure 2. Jurkat cells were stained with intracellular CD3 followed by goat anti-mouse IgG conjugates. Background staining was determined by secondary antibody staining alone. Fluorescence was analyzed on a BD FACS Calibur in the FL4 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.   Figure 3. Photostability comparison between CF™640R and Atto™647N, a far-red dye frequently used in single-molecule imaging. Dye solutions at 0.5 mM in PBS were continuously exposed using a Cy™ 5 filter set on a mercury arc lamp microscope. Images were captured every 15 seconds, and mean fluorescence intensity of each image was calculated using Image Pro Express software.

Table A. CF640R Dye-labeled Secondary Antibody Conjugates

Cat#
Product Name
Unit Size
Unit Price
20291 Bovine serum albumin, CF640R conjugate 5x1mg $170
20297 CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $180
20297-1 CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20179 CF640R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL 0.5 mL $180
20179-1 CF640R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL 50 uL $75
20177 CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL 0.5 mL $180
20177-1 CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL 50 uL $75
20178 CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL 0.5 mL $180
20178-1 CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL 50 uL $75
20199 CF640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $160
20199-1 CF640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $65
20083 CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL 0.5 mL $180
20083-1 CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL 50 uL $75
20084 CF640R Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $160
20084-1 CF640R Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20085 CF640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL 0.5 mL $150
20085-1 CF640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL 50 uL $60
20081 CF640R Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $180
20081-1 CF640R Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20197 CF640R Goat anti-mouse IgG (H+L), 2 mg/mL 0.5 mL $150
20197-1 CF640R Goat anti-mouse IgG (H+L), 2 mg/mL 50 uL $60
20086 CF640R Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL 0.25 mL $105
20304 CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL 0.5 mL $235
20304-1 CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL 50 uL $100
20175 CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $180
20175-1 CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20251 CF640R Goat anti-mouse IgG1, (γ1), 2 mg/mL 0.25 mL $195
20261 CF640R Goat anti-mouse IgG2a, (γ2a), 2mg/mL 0.25 mL $195
20271 CF640R Goat anti-mouse IgG2b, (γ2b), 2mg/mL 0.25 mL $195
20202 CF640R Goat Anti-Rabbit IgG (H+L), 2mg/mL 0.5 mL $150
20202-1 CF640R Goat Anti-Rabbit IgG (H+L), 2mg/mL 50 uL $60
20087 CF640R Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL 0.25 mL $105
20176 CF640R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $180
20176-1 CF640R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20088 CF640R Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $180
20088-1 CF640R Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $75
20089 CF640R Goat Anti-Swine IgG (H+L), 2 mg/mL 0.5 mL $150
20089-1 CF640R Goat Anti-Swine IgG (H+L), 2 mg/mL 50 uL $60
20237 CF640R Monoclonal Mouse Anti-6X His IgG, 1mg/mL 50 uL $285
20207 CF640R Monoclonal Mouse Anti-Biotin, 2 mg/mL 0.25 mL $180
20207-1 CF640R Monoclonal Mouse Anti-Biotin, 2 mg/mL 50 uL $85
20218 CF640R monoclonal mouse anti-GFP IgG, 1 mg/mL 0.1 mL $220
20090 CF640R Rabbit Anti-Goat IgG (H+L), 2 mg/mL 0.5 mL $150
20090-1 CF640R Rabbit Anti-Goat IgG (H+L), 2 mg/mL 50 uL $60
20200 CF640R Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL 0.5 mL $160
20200-1 CF640R Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL 50 uL $65
20201 CF640R Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 0.5 mL $160
20201-1 CF640R Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL 50 uL $65

Table B. Other CF640R dye-labeled Products

Cat#
Product Name
Unit Size
Unit Price
29014 Annexin V, CF640R conjugate, 50 ug/mL 0.5 mL $220
29019 CF640R Concanavalin A (Con A) 5x1 mg $130
29026 Wheat Germ Agglutinin, CF640R conjugate 5x1mg $250
29026-1 Wheat Germ Agglutinin, CF640R conjugate 1mg $60

Table C. Reactive CF640R Dyes and Labeling Kits

Cat#
Product Name
Unit Size
Unit Price
92225 CF640R SE protein labeling kit 3 labelings $365
92108 CF640R succinimidyl ester 1 umole $225
92258 Mix-n-Stain CF640R antibody labeling kit, 1x(20-50 ug) labeling 1 labeling $105
92245 Mix-n-Stain CF640R antibody labeling kit, 1x(50-100 ug) labeling 1 labeling $115
92278 Mix-n-Stain CF640R antibody labeling kit, 1X(5-20ug) labeling 1 labeling $95
**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

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