Near-IR CF™ Dyes
Simply the brightest and most stable long wavelength dyes

Developed by scientists at Biotium, Near-IR CF dyes are a group of fluorescent dyes with absorption and emission wavelengths between 650 and 800 nm. Designed for protein labeling, near-IR CF dyes are significantly brighter and more stable than any other commercial dye of similar wavelengths. Near-IR dyes offer important advantages over traditional visible light dyes. Because cellular or tissue components produce minimal autofluorescence in the near-IR region, near-IR dyes have the potential to offer highly specific and sensitive detection in complex biological systems. Also, because light with wavelength in the near-IR region has strong tissue penetration, near-IR dyes are ideal for in vivo fluorescence imaging, an emerging field that has advanced rapidly in recent years. Futhermore, near-IR dyes are also excellent dyes for in- or on-cell and membrane-based Western assays.
Near-IR CF dyes are next-generation long wavelength dyes representing a true breakthrough in the field. The current commercial near-IR dyes suffer from problems of limited fluorescence brightness due to excessive dye aggregation and poor stability. As a result of novel molecular engineering by scientists at Biotium, near-IR CF dyes overcome these problems, resulting in several key advantages over other competitive dyes. Near-IR CF dyes are exceptionally bright and stable. For example, our CF750 is so bright, it can be excited at 633 nm (i.e., at the shoulder wavelength of the absorption maximum) but still emits stronger fluorescence at ~770 nm than APC-based tandem dyes, making the dye particularly useful for flow cytometry applications without the spillover and stability challenges encountered with tandem dyes.

In addition, near-IR CF dyes possess a proprietary structural feature that renders the dyes less immunogenic than other competitive dyes. Consequently, a higher number of near-IR CF dyes can be conjugated to a protein for maximal fluorescence, and the resulting protein conjugate can be expected to have a longer in vivo half-life. Finally, near-IR CF dyes have much higher labeling efficiency than other near-IR dyes because of their excellent solubility and generally >95% reactivity

ADVANTAGES

• Brightness: CF dyes match or surpass the brightness of Alexa Fluor, Cy and other commercially available dyes.
• Photostability: CF dyes are among the most photostable dyes.
• Specificity and in-vivo Stability: CF-labeled conjugates offer super specificity and improved half-life for in-vivo imaging.
• pH Insensitivity: CF dyes remain highly fluorescent over a wide pH range.
• Solubility: CF dyes are highly soluble in both water and a wide range of organic solvents.
• Compatibility: CF dyes are spectrally similar to other commercially available dyes and compatible with common excitation sources and existing filters.
• Color Selection: CF dye series offers the largest dye collection with wavelengths ranging from UV to near IR.

APPLICATIONS

• In Vivo Imaging: CF reactive dyes and kits for small animal imaging
• Westerns: Perform Westerns with highly cross-adsorbed antibodies to minimize cross-reactivity and background
• Protein Labeling: Reactive dyes and kits for custom conjugations
• Flow Cytometry: Superior non-tandem conjugates for cell staining
• Microscopy: Bright and photostable antibodies or reactive dyes for multiple labeling procedures

Table A. Near-IR CF Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20292	Bovine serum albumin, CF680 conjugate	5x1mg	$190
20060	CF680 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$175
20060-1	CF680 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20241	CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20241-1	CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20278	CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20278-1	CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20062	CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$175
20062-1	CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20287	CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20287-1	CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20065	CF680 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL	0.25 mL	$175
20063	CF680 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$145
20065-1	CF680 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20253	CF680 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$210
20263	CF680 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$210
20273	CF680 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$210
20067	CF680 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL	0.25 mL	$180
20064	CF680 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$145
20067-1	CF680 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20069	CF680 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$175
20069-1	CF680 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20219	CF680 monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$240
20068	CF680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$165
20068-1	CF680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$70
20243	CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$175
20243-1	CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$70
20061	CF680 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.25 mL	$175
20061-1	CF680 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20196	CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20196-1	CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20194	CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20194-1	CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20195	CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20195-1	CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20192	CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20192-1	CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20193	CF680R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20193-1	CF680R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20298	CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20298-1	CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20070	CF750 Goat Anti-Mouse IgG (H+L), 2 mg/mL	0.5 mL	$165
20070-1	CF750 Goat Anti-Mouse IgG (H+L), 2 mg/mL	50 uL	$70
20073	CF750 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$165
20073-1	CF750 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$70
20277	CF770 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20277-1	CF770 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20242	CF770 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20242-1	CF770 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20279	CF770 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20279-1	CF770 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20288	CF770 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20288-1	CF770 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20077	CF770 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL	0.25 mL	$175
20077-1	CF770 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL	50 uL	$75
20254	CF770 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$210
20264	CF770 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$210
20274	CF770 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$210
20078	CF770 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL	0.25 mL	$175
20078-1	CF770 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL	50 uL	$75
20220	CF770 monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$240
20244	CF770 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$175
20244-1	CF770 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$70

Table B. Other Near-IR CF dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29007	Annexin V, CF680 conjugate, lyophilized	25 ug	$250
29006	Annexin V, CF750 conjugate, lyophilized	25 ug	$250
00048	Phalloidin, CF680R conjugate	300 U	$325
29025	Wheat Germ Agglutinin, CF680R conjugate	5x1mg	$270
29025-1	Wheat Germ Agglutinin, CF680R conjugate	1mg	$70

Table C. Reactive Near-IR CF Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92029	CF680 maleimide	1 umole	$205
92220	CF680 SE protein labeling kit	3 labelings	$365
92139	CF680 succinimidyl ester	1 umole	$240
92032	CF680R maleimide	1 umole	$230
92226	CF680R SE protein labeling kit	3 labelings	$365
92107	CF680R succinimidyl ester	1 umole	$250
92221	CF750 SE protein labeling kit	3 labelings	$365
92142	CF750 succinimidyl ester	1 umole	$240
92222	CF770 SE protein labeling kit	3 labelings	$365
92150	CF770 succinimidyl ester	1 umole	$240
92155	CF790 succinimidyl ester	0.25 umole	$140
92282	Mix-n-Stain CF680 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92283	Mix-n-Stain CF680R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92241	Mix-n-Stain CF750 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92284	Mix-n-Stain CF750 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92265	Mix-n-Stain CF770 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92242	Mix-n-Stain CF770 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92285	Mix-n-Stain CF770 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92268	Mix-n-Stain CF790 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92248	Mix-n-Stain CF790 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92288	Mix-n-Stain CF790 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92160	VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF680 SE	3 labelings	$395
92161	VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF750 SE	3 labelings	$395
92162	VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF770 SE	3 labelings	$395
92163	VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF790 SE	3 labelings	$410

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™680 and CF™680R Dyes
Two outstanding 680 nm-excitable dyes to meet different needs

CF™680 and CF™680R are two outstanding near-IR dyes excitable at about 680 nm with emission at about 700 nm. The two dyes each have unique properties suitable for different application needs. CF™680 is a highly water-soluble cyanine-based dye with a molecular weight of ~3000. This dye is excellent for labeling antibodies, producing the brightest fluorescence and highest signal-to-noise ratio in immunostaining among spectrally similar dyes, such as Cy™5.5, Alexa Fluor™ 680, DyLight™ 680 and IRDye™ 680. However, because of its relatively large molecular size, CF™680 is not recommended for labeling nucleic acids or relatively small biomolecules, for which our CF™680R is better suited.
CF™680R is a novel rhodamine-based dye with a relatively small molecular weight of 912. The dye is highly fluorescent and, more importantly, extremely photostable. Rhodamine dyes are traditionally known to be bright and photostable. However, it has been a synthetic challenge to make functional rhodamine dyes with long wavelengths and high water solubility necessary for bio-labeling. Scientists at Biotium have overcome the challenge and invented a new way to make highly water soluble, bright and photostable rhodamine dyes with wavelengths ranging from red to near-IR. These dyes are excellent for labeling proteins, nucleic acids and small
bio-molecules. They are ideal for confocal microscopy, single moleculebased imaging and other applications that demand both brightness and photostability.
A list of CF™680- and CF™680R-based products are shown in Table 1. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary CF™680 Abs/Em Maxima: 681/698 nm Extinction coefficient: 210,000 Molecular weight: 3241 Direct replacement for: Alexa Fluor® 680, Cy™5.5		Advantages CF™680 The brightest among spectrally similar 680 nm dyes Superior signal-to-noise ratio in immunostaining Highly water-soluble and pH-insensitive
CF™680R Abs/Em Maxima: 680/701 nm Extinction coefficient: 140,000 Molecular weight: 912 Direct replacement for: Alexa Fluor® 680, Cy™5.5		CF™680R The most photostable 680 nm dye suitable for labeling nucleic acids and small bio-molecules Highly water-soluble and pH-insensitive
Figure 1. Absorption and emission spectra of CF™680 and CF™680R conjugated to goat anti-mouse IgG, respectively, in PBS.		Figure 2. Jurkat cells were stained with isotype or mouse anti-human intracellular CD3 antibody followed by 1 mg of goat anti-mouse IgG conjugated with Cy™5.5, AlexaFluor® 680, CF™680 or DyLight™680. Fluorescence was detected by a BD FACS Calibur in the FL4 channel. The bars represent the signal-to-noise ratio of CD3 positive fluorescence to isotype using similar degrees of labeling (DOL).
Figure 3. Photostability comparison by microscopy. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™680R (Biotium), AlexaFluor® 680 (Invitrogen) or Cy™5.5 (GE Healthcare) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0, 1 min and 5 min.		Figure 4. Photostability comparison by microscopy. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™680R, CF™F680, Cy™5.5 or AlexaFluor® 680 (AF680) goat anti-mouse IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. The graph illustrates the relative fluorescence intensities of sequential images taken every 10 seconds for 5 minutes.
Figure 5. HeLa cells were stained with mouse a-tubulin antibody followed by CF™680 goat anti-mouse IgG. Images were captured using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set, CCD camera and ImagePro Express software.		Figure 6. Near-IR CF™680 and CF™770 for two-color Western blotting. Two-fold dilutions of HeLa cell lysate were run on an acrylamde gel, transferred to a nitrocellulose membrane and probed with mouse alpha-tubulin and rabbit COX IV primary antibodies followed by goat antimouse CF™770 or IRDye™ 800 (green) and goat anti-rabbit CF™680 or IRDye™680 (red) at the same final concentrations. After probing, membranes were dried and scanned using an Odyssey® infrared imaging system (Li-Cor Biosciences). Quantitation of the bands showed approximately a 3.5-fold higher fluorescence intensity of CF dyes compared to the respective IRDye™ secondary antibodies (Li-Cor).

Table A. CF680 and CF680R Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20292	Bovine serum albumin, CF680 conjugate	5x1mg	$190
20060	CF680 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$175
20060-1	CF680 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20241	CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20241-1	CF680 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20278	CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20278-1	CF680 donkey anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20062	CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$175
20062-1	CF680 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20287	CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$180
20287-1	CF680 goat anti-human IgG(H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20065	CF680 Goat Anti-Mouse IgG (H+L) (min X Human, Bovine, Horse, Rabbit, and Swine), 2 mg/mL	0.25 mL	$175
20063	CF680 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$145
20065-1	CF680 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20253	CF680 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$210
20263	CF680 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$210
20273	CF680 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$210
20067	CF680 Goat Anti-Rabbit IgG (H+L) (min X Human, Mouse, and Rat), 2 mg/mL	0.25 mL	$180
20064	CF680 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$145
20067-1	CF680 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20069	CF680 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$175
20069-1	CF680 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20219	CF680 monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$240
20068	CF680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$165
20068-1	CF680 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$70
20243	CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$175
20243-1	CF680 Rabbit Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$70
20061	CF680 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.25 mL	$175
20061-1	CF680 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20196	CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20196-1	CF680R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20194	CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20194-1	CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20195	CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.25 mL	$185
20195-1	CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20192	CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20192-1	CF680R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20193	CF680R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.25 mL	$185
20193-1	CF680R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75

Table B. Other CF680 and CF680R dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29007	Annexin V, CF680 conjugate, lyophilized	25 ug	$250
00048	Phalloidin, CF680R conjugate	300 U	$325
29025	Wheat Germ Agglutinin, CF680R conjugate	5x1mg	$270
29025-1	Wheat Germ Agglutinin, CF680R conjugate	1mg	$70

Table C. Reactive CF680 and CF680R Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92029	CF680 maleimide	1 umole	$205
92220	CF680 SE protein labeling kit	3 labelings	$365
92139	CF680 succinimidyl ester	1 umole	$240
92032	CF680R maleimide	1 umole	$230
92226	CF680R SE protein labeling kit	3 labelings	$365
92107	CF680R succinimidyl ester	1 umole	$250
92282	Mix-n-Stain CF680 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92283	Mix-n-Stain CF680R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92160	VivoBrite rapid antibody labeling kit for small animal in vivo imaging, CF680 SE	3 labelings	$395

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™660C and CF™660R Dyes
Superior alternatives to Alexa Fluor® 660

CF™660C and CF™660R are two spectrally similar fluorescent dyes that emit fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR (Figure 1). Although their absorption maxima are at around 660 nm, both dyes can be sufficiently excited by the 633 or 635 nm laser. When combined with other CF™ dyes of shorter wavelengths, CF™660C or CF™660R can serve as a useful long wavelength dye in multi-color detection applications. The two dyes are spectrally similar to Alexa Fluor® 660 but are far superior to the latter in performance. Like Alexa Fluor® 660, CF™660C is a cyanine-based dye. However, when conjugated to protein, CF™660C is several fold brighter and significantly more photostable than Alexa Fluor® 660 (Figures 2 and 3). CF™660R is a rhodamine-based dye. Like rhodamine dyes in general, CF™660R is exceptionally photostable, compared to both Alexa Fluor® 660 and CF™660C (Figure 2). CF™660R is also much brighter than Alexa Fluor® 660, though not as bright as CF™660C (Figure 2). The superior photostability and excellent brightness of CF™660R make the dye an ideal choice for confocal microscopy and other demanding applications.
A list of CF™660C- and CF™660R-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary CF™660C Abs/Em Maxima: 667/685 nm Extinction coefficient: 200,000 Molecular weight: 3112 Flow cytometry laser line: 633 or 635 nm Microscopy laser line: 633 or 635 nm Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC) CF™660R Abs/Em Maxima: 663/682 nm Extinction coefficient: 100,000 Molecular weight: 888 Flow cytometry laser line: 633 or 635 nm Microscopy laser line: 633 or 635 nm Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC) Advantages of CF™660C: Much brighter than Alexa Fluor® 660 More photostable than Alexa Fluor® 660 Highly water-soluble Advantages of CF™660R: Brighter than Alexa Fluor® 660 The most photostable 660 nm dye ideal for confocal microscopy application Highly water-soluble
		Figure 1. Absorption and emission spectra of CF™660C and CF™660R conjugated to goat anti-mouse IgG, respectively, in PBS.
		Figure 2. Relative fluorescence of CF™660C-, CF™660R and Alexa Fluor® 660-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 633 nm excitation.
Figure 3. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™660C (Biotium) or AlexaFluor® 660 (Invitrogen) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0 and 10 second.

 

Table A. CF660 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20051	CF660C Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20051-1	CF660C Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20050	CF660C Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20050-1	CF660C Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20052	CF660C Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20052-1	CF660C Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20053	CF660C Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20053-1	CF660C Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20054	CF660R Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20054-1	CF660R Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20055	CF660R Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20055-1	CF660R Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60

Table B. Other CF660 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29040	CF660R Streptavidin	1 mg	$160
00047	Phalloidin, CF660R conjugate	300 U	$305

Table C. Reactive CF660 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92219	CF660C SE protein labeling kit	3 labelings	$365
92137	CF660C succinimidyl ester	1 umole	$225
92223	CF660R SE protein labeling kit	3 labelings	$365
92134	CF660R succinimidyl ester	1 umole	$225
92239	Mix-n-Stain CF660C antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92280	Mix-n-Stain CF660C antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95
92243	Mix-n-Stain CF660R antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92281	Mix-n-Stain CF660R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™647 Dye
A far-red dye superior to Cy™5 and Alexa Fluor® 647

CF™647 is a cyanine-based far-red fluorescent dye spectrally similar to Cy™5 and Alexa Fluor® 647 (Figure 1). Compared with the other dyes, CF™647 has good fluorescence brightness (Figures 2 and 3A). However, the most attractive feature of CF™647 is its superior signal-to-noise ratio for its antibody conjugates in immunostaining (Figure 3B). Alexa Fluor® 647 bears multiple negative charges, which improve the brightness of the dye but also significantly alter the isoelectric point of antibody conjugates, resulting in lowered specificity of the conjugates. To overcome the problem, a polymer blocking agent, such as Image-it™, is recommended to pre-treat the sample prior to the staining. CF™647, on the other hand, is minimally charged. As a result, antibody conjugates prepared from the dye have excellent specificity.
A list of CF™647-based products are shown in Table 1. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 650/665 nm Extinction coefficient: 240,000 Molecular weight: ~2980 Flow cytometry laser line: 633 or 635 nm Microscopy laser line: 633 or 635 nm Direct replacement for: Cy™5, Alexa Fluor® 647 and DyLight™ 649 Advantages • Yields antibody conjugates having the best signal-to-noise ratio compared to Alexa Fluor® 647, DyLight™ 649 and Cy™ 5 • Bright • Highly water-soluble • pH-insensitive
	Figure 1. Absorption and emission spectra of CF™647 conjugated to goat anti-mouse IgG in PBS.	Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis and stained with NucView™488 (live cell caspase-3 detection) and CF™647 Annexin-V. CF™647 Annexin-V staining is pseudocolored magenta.
	Figure 3. Jurkat cells were fixed, permeabilized and stained with intracellular mouse anti-human CD3 followed by CF™647 (Biotium), AlexaFluor® 647 (Invitrogen) or other commercially available goat anti-mouse IgG conjugates. Stained cells were analyzed on a BD FACS Calibur in the FL4 channel. (A) The bars represent the relative geometric means of the cell population. Background was determined by secondary antibody staining alone. (B) The signal-to-noise ratio of the geometric means from A.

Table A. CF647 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20048	CF647 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL	0.5 mL	$180
20048-1	CF647 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL	50 uL	$75
20046	CF647 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL	0.5 mL	$180
20046-1	CF647 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL	50 uL	$75
20047	CF647 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL	0.5 mL	$180
20047-1	CF647 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL	50 uL	$75
20284	CF647 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20284-1	CF647 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20042	CF647 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL	0.25 mL	$120
20045	CF647 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.25 mL	$120
20044	CF647 Goat Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20044-1	CF647 Goat Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20041	CF647 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20041-1	CF647 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20280	CF647 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20280-1	CF647 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20040	CF647 Goat Anti-Mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20040-1	CF647 Goat Anti-Mouse IgG (H+L), 2 mg/mL	50 uL	$60
20281	CF647 Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20281-1	CF647 Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20252	CF647 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20262	CF647 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20272	CF647 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20043	CF647 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20043-1	CF647 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20282	CF647 Goat Anti-Rabbit IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20282-1	CF647 Goat Anti-Rabbit IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20283	CF647 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20283-1	CF647 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20286	CF647 Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20286-1	CF647 Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20049	CF647 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20049-1	CF647 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20285	CF647 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20285-1	CF647 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75

Table B. Other CF647 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29003	Annexin V, CF647 conjugate, 50ug/mL	0.5 mL	$220
00041	Phalloidin, CF647 conjugate	300 U	$305

Table C. Reactive CF647 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92218	CF647 SE protein labeling kit	3 labelings	$365
92135	CF647 succinimidyl ester	1 umole	$225
92279	Mix-n-Stain CF647 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™640R Dye
A highly photostable far-red dye for the 640 nm diode laser

CF™640R is a rhodamine-based far-red fluorescent dye with excitation and emission maxima very similar to those of Cy™5 and Alexa Fluor® 647 (Figure 1). CF™640R is much brighter than Cy™5 and at least as bright as Alexa Fluor® 647 (Figure 2). A major advantage of CF™640R over Cy™ 5 and Alexa Fluor® 647 is its exceptional photostability. Cy™5 and Alexa Fluor® 647 are cyanine-based dyes and, like other cyanine dyes in general, have intrinsically poor photostability. CF™640R is also superior to Atto™ 647N, another spectrally similar dye frequently used in single-molecule imaging. As shown in Figures 2 and 3, CF™640R is significantly brighter and more photostable than the Atto™ dye. The combination of excellent brightness and photostability makes CF™640R ideal for confocal microscopy, single- molecule imaging and other demanding applications based on fluorescence detection.
A list of CF™640R-based products are shown in Table 1. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 642/662 nm Extinction coefficient: 105,000 Molecular weight: 832 Flow cytometry laser line: 633, 635 or 640 nm Microscopy laser line: 633, 635 or 640 nm Direct replacement for: Cy™5, Alexa Fluor® 647 and ATTO™ 647N Advantages Has the best photostability among dyes with Cy™5-like spectra Yields highly fluorescent protein conjugates Very water-soluble and pH-insensitive
		Figure 1. Absorption and emission spectra of CF™640R and Cy™5 conjugated to goat anti-mouse IgG in PBS.
Figure 2. Jurkat cells were stained with intracellular CD3 followed by goat anti-mouse IgG conjugates. Background staining was determined by secondary antibody staining alone. Fluorescence was analyzed on a BD FACS Calibur in the FL4 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.		Figure 3. Photostability comparison between CF™640R and Atto™647N, a far-red dye frequently used in single-molecule imaging. Dye solutions at 0.5 mM in PBS were continuously exposed using a Cy™ 5 filter set on a mercury arc lamp microscope. Images were captured every 15 seconds, and mean fluorescence intensity of each image was calculated using Image Pro Express software.

Table A. CF640R Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20291	Bovine serum albumin, CF640R conjugate	5x1mg	$170
20297	CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20297-1	CF640R Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20179	CF640R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20179-1	CF640R Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20177	CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20177-1	CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20178	CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20178-1	CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20199	CF640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$160
20199-1	CF640R Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$65
20083	CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20083-1	CF640R Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20084	CF640R Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$160
20084-1	CF640R Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20085	CF640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20085-1	CF640R Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20081	CF640R Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20081-1	CF640R Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20197	CF640R Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20197-1	CF640R Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20086	CF640R Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$105
20304	CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	0.5 mL	$235
20304-1	CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	50 uL	$100
20175	CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20175-1	CF640R Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20251	CF640R Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20261	CF640R Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20271	CF640R Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20202	CF640R Goat Anti-Rabbit IgG (H+L), 2mg/mL	0.5 mL	$150
20202-1	CF640R Goat Anti-Rabbit IgG (H+L), 2mg/mL	50 uL	$60
20087	CF640R Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$105
20176	CF640R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20176-1	CF640R Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20088	CF640R Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20088-1	CF640R Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20089	CF640R Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20089-1	CF640R Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20237	CF640R Monoclonal Mouse Anti-6X His IgG, 1mg/mL	50 uL	$285
20207	CF640R Monoclonal Mouse Anti-Biotin, 2 mg/mL	0.25 mL	$180
20207-1	CF640R Monoclonal Mouse Anti-Biotin, 2 mg/mL	50 uL	$85
20218	CF640R monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$220
20090	CF640R Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20090-1	CF640R Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20200	CF640R Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$160
20200-1	CF640R Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$65
20201	CF640R Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$160
20201-1	CF640R Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$65

Table B. Other CF640R dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29014	Annexin V, CF640R conjugate, 50 ug/mL	0.5 mL	$220
29019	CF640R Concanavalin A (Con A)	5x1 mg	$130
29026	Wheat Germ Agglutinin, CF640R conjugate	5x1mg	$250
29026-1	Wheat Germ Agglutinin, CF640R conjugate	1mg	$60

Table C. Reactive CF640R Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92225	CF640R SE protein labeling kit	3 labelings	$365
92108	CF640R succinimidyl ester	1 umole	$225
92258	Mix-n-Stain CF640R antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92245	Mix-n-Stain CF640R antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92278	Mix-n-Stain CF640R antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™633 Dye
Truly the best dye for the 633/635 nm lasers

Far-red fluorescent dyes offer the advantage of ultra sensitive detection because background signal due to autofluorescence in most biological samples is minimal in this spectral region. For many years, the cyanine dye Cy™5 has been the dye of choice for such detection. More recently, Alexa Fluor^® 647 has been developed as a better alternative by having brighter fluorescence and higher photostability. Despite the improvement, Alexa Fluor^®647 still lacks sufficient photostability required for many demanding applications. On the other hand, while Alexa Fluor^® 633 is photostable, its fluorescence on proteins is very weak. In fact, it has been a challenging task for dye chemists to develop a far-red fluorescent dye that is both highly fluorescent and photostable for protein and nucleic acid labeling. Using new chemistry, scientists at Biotium have successfully developed CF™633 to overcome these challenges. With its absorption peak at 630 nm, CF™633 is optimally excited by the 633 nm He-Ne laser or the 635 nm red diode laser. Its emission maximum is at 650 nm, which is 15 nm shorter than that of Alexa Fluor^® 647 or Cy™5. Although the detection window on most flow cytometers is centered around the emission peak wavelength of Alexa Fluor® 647 or other Cy™5-like cyanine dyes, CF™633 is still significantly brighter (Figure 2). The most important advantage of CF™633, however, is its unmatched photostability (Figure 3). The combination of superior brightness and photostability make CF™633 the best choice for any detection system using a 633 or 635 nm laser excitation source.

A list of CF™633-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidin, annexin V and a-bungarotoxin are also available for many CF™ dyes. Please visit the Biotium website at www.biotium.com for details.

Technical Summary Abs/Em Maxima: 630/650 nm Extinction coefficient: 100,000 Molecular weight: ~820 Flow cytometry laser line: 633 or 635 nm Microscopy laser line: 633 or 635 nm Direct replacement for: Alexa Fluor® 633, Alexa Fluor® 647, Cy™5 and DyLight™ 649 Advantages Yields the brightest antibody conjugates among spectrally similar dyes when excited by the 633 nm He-Ne laser or the 635 nm red diode laser Far more photostable than Alexa Fluor® 647 Highly water-soluble

Figure 1. Absorption and emission spectra of CF™633 conjugated to goat anti-mouse IgG in PBS.

Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL4 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.		Figure 3. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™633 (Biotium) or AlexaFluor® 647 (Invitrogen) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0, 1 min and 5 min.

Table A. CF633 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20296	CF633 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20296-1	CF633 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20227	CF633 Chicken Anti-Goat IgG (H+L), 2mg/mL	0.5 mL	$150
20227-1	CF633 Chicken Anti-Goat IgG (H+L), 2mg/mL	50 uL	$65
20222	CF633 Chicken Anti-Mouse IgG (H+L), 2mg/mL	0.5 mL	$150
20222-1	CF633 Chicken Anti-Mouse IgG (H+L), 2mg/mL	50 uL	$65
20224	CF633 Chicken Anti-Rabbit IgG (H+L), 2mg/mL	0.5 mL	$150
20224-1	CF633 Chicken Anti-Rabbit IgG (H+L), 2mg/mL	50 uL	$65
20168	CF633 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20168-1	CF633 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20127	CF633 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20127-1	CF633 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20171	CF633 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20171-1	CF633 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20076	CF633 Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20076-1	CF633 Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20124	CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20124-1	CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20125	CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20125-1	CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20137	CF633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20137-1	CF633 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20134	CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20134-1	CF633 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20126	CF633 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20126-1	CF633 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20129	CF633 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20129-1	CF633 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20132	CF633 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20132-1	CF633 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20120	CF633 Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20120-1	CF633 Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20130	CF633 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20121	CF633 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$185
20121-1	CF633 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20250	CF633 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20260	CF633 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20270	CF633 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20122	CF633 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20122-1	CF633 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20131	CF633 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20123	CF633 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20123-1	CF633 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20133	CF633 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20133-1	CF633 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20138	CF633 Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20138-1	CF633 Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20206	CF633 Monoclonal Mouse Anti-Biotin, 2 mg/mL	0.25 mL	$180
20206-1	CF633 Monoclonal Mouse Anti-Biotin, 2 mg/mL	50 uL	$85
20217	CF633 monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$220
20165	CF633 Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	0.5 mL	$150
20165-1	CF633 Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	50 uL	$60
20128	CF633 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20128-1	CF633 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20066	CF633 Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$160
20066-1	CF633 Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$65
20136	CF633 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20136-1	CF633 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20135	CF633 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20135-1	CF633 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20174	CF633 Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20174-1	CF633 Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75

Table B. Other CF633 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29008	Annexin V, CF633 conjugate, 50 ug/mL	0.5 mL	$220
00009	CF633 a-Bungarotoxin	0.5 mg	$300
29018	CF633 Concanavalin A (Con A)	5x1 mg	$130
29037	CF633 Streptavidin	1 mg	$160
00046	Phalloidin, CF633 conjugate	300 U	$305
29024	Wheat Germ Agglutinin, CF633 conjugate	5x1mg	$250
29024-1	Wheat Germ Agglutinin, CF633 conjugate	1mg	$60

Table C. Reactive CF633 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92156	CF633 hydrazide	1 mg	$150
92026	CF633 maleimide	1 umole	$205
92217	CF633 SE protein labeling kit	3 labelings	$365
92133	CF633 succinimidyl ester	1 umole	$225
92053	CF633, aminooxy	1 mg	$390
92257	Mix-n-Stain CF633 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92237	Mix-n-Stain CF633 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92277	Mix-n-Stain CF633 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™594 Dye
Truly the brightest deep-red dye

CF™594 is a deep red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye (Figure 1). On protein, CF™594 is significantly brighter than Alexa Fluor® 594 due to its high quantum yield and exceptional water solubility (Figure 2). CF™594 also has excellent photostability ideal for demanding applications, such as confocal and single molecular imagings (Figure 3). These properties make CF™594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful to combine with our blue fluorescent CF™350, green fluorescent CF™488A and far red CF™647 for multi-color imaging.
A list of CF™594-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 593/614 nm Extinction coefficient: 115,000 Molecular weight: ~730 Flow cytometry laser line: 532 or 594 nm Microscopy laser line: 594 nm Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight 594 and Cy™3. Advantages Yields the brightest antibody conjugates among spectrally similar dyes. Extremely photostable Highly water-soluble
	Figure 1. Absorption and emission spectra of CF™594 conjugated to goat anti-mouse IgG in PBS.
Figure 3. Jurkat cells were stained with intracellular CD3 followed by CF594 or AlexaFluor594 goat anti-mouse conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.
	Figure 2. Relative fluorescence of CF™594- and Alexa Fluor® 594-goat anti-mouse conjugates as a function of number of dye per protein or degree of labeling (DOL). Fluorescence was measured at each dye’s emission maximum in PBS using 594 nm excitation.

Table A. CF594 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20290	Bovine serum albumin, CF594 conjugate	5x1mg	$170
20295	CF594 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20295-1	CF594 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20226	CF594 Chicken Anti-Goat IgG (H+L), 2mg/mL	0.5 mL	$150
20226-1	CF594 Chicken Anti-Goat IgG (H+L), 2mg/mL	50 uL	$65
20221	CF594 Chicken Anti-Mouse IgG (H+L), 2mg/mL	0.5 mL	$150
20221-1	CF594 Chicken Anti-Mouse IgG (H+L), 2mg/mL	50 uL	$65
20223	CF594 Chicken Anti-Rabbit IgG (H+L), 2mg/mL	0.5 mL	$150
20223-1	CF594 Chicken Anti-Rabbit IgG (H+L), 2mg/mL	50 uL	$65
20167	CF594 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20167-1	CF594 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20116	CF594 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20116-1	CF594 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20170	CF594 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20170-1	CF594 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20075	CF594 Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20075-1	CF594 Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20115	CF594 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20115-1	CF594 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20152	CF594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20152-1	CF594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20159	CF594 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20159-1	CF594 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20156	CF594 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20156-1	CF594 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20114	CF594 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20114-1	CF594 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20118	CF594 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20118-1	CF594 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20154	CF594 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20154-1	CF594 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20110	CF594 Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20110-1	CF594 Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20119	CF594 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20303	CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	0.5 mL	$235
20303-1	CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	50 uL	$100
20111	CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20111-1	CF594 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20249	CF594 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20259	CF594 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20269	CF594 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20112	CF594 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20112-1	CF594 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20153	CF594 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20113	CF594 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20113-1	CF594 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20155	CF594 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20155-1	CF594 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20160	CF594 Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20160-1	CF594 Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20229	CF594 Monoclonal Mouse Anti-6X His IgG, 1mg/mL	50 uL	$285
20205	CF594 Monoclonal Mouse Anti-Biotin, 2 mg/mL	0.25 mL	$180
20205-1	CF594 Monoclonal Mouse Anti-Biotin, 2 mg/mL	50 uL	$85
20216	CF594 monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$220
20164	CF594 Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	0.5 mL	$150
20164-1	CF594 Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	50 uL	$60
20117	CF594 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20117-1	CF594 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20072	CF594 Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20072-1	CF594 Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20158	CF594 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20158-1	CF594 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20157	CF594 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20157-1	CF594 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20173	CF594 Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20173-1	CF594 Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75

Table B. Other CF594 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29011	Annexin V, CF594 conjugate, 50 ug/mL	0.5 mL	$220
00007	CF594 a-Bungarotoxin	0.5 mg	$300
29017	CF594 Concanavalin A (Con A)	5x1 mg	$130
29036	CF594 Streptavidin	1 mg	$160
00045	Phalloidin, CF594 conjugate	300 U	$305
29023	Wheat Germ Agglutinin, CF594 conjugate	5x1mg	$250
29023-1	Wheat Germ Agglutinin, CF594 conjugate	1mg	$60

Table C. Reactive CF594 Dyes and Labeling Kits

CF594 hydrazide

Cat#	Product Name	Unit Size	Unit Price
92158	CF594 hydrazide	1 mg	$150
92025	CF594 maleimide	1 umole	$205
92216	CF594 SE protein labeling kit	3 labelings	$365
92132	CF594 succinimidyl ester	1 umole	$225
92052	CF594, aminooxy	1 mg	$390
92236	Mix-n-Stain CF594 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92276	Mix-n-Stain CF594 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™568 Dye
A far superior red dye to Alexa Fluor® 568

CF™568 is a red fluorescent dye with an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser (Figure 1). Antibody conjugates of the dye are much brighter than those of Alexa Fluor® 568. In addition, the photostability of CF™568 is superior to that of Alexa Fluor® 568, making CF™568 a much better choice for demanding applications, such as confocal and single molecular imagings.
A list of CF™568-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 562/583 nm Extinction coefficient: 100,000 Molecular weight: 714 Flow cytometry laser line: 532 nm Microscopy laser line: 568 nm Direct replacement for: Alexa Fluor® 568, Lissamine rhoda- mine B, ROX Advantages Yields much brighter antibody conjugates than Alexa Fluor® 568 Extremely photostable Highly water-soluble
	Figure 1. Absorption and emission spectra of CF™568 conjugated to goat anti-mouse IgG in PBS.
	Figure 3. Jurkat cells were stained with intracellular biotin-CD3 followed by streptavidin CF568 or AlexaFluor568 conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.
Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL2 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.

Table A. CF568 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20294	CF568 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20294-1	CF568 Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20106	CF568 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20106-1	CF568 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20105	CF568 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20105-1	CF568 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20098	CF568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20098-1	CF568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20092	CF568 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20092-1	CF568 Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20095	CF568 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20095-1	CF568 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20104	CF568 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20104-1	CF568 Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20108	CF568 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20108-1	CF568 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20097	CF568 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20097-1	CF568 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20100	CF568 Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20100-1	CF568 Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20109	CF568 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20301	CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	0.5 mL	$235
20301-1	CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	50 uL	$100
20101	CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20101-1	CF568 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20248	CF568 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20258	CF568 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20268	CF568 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20102	CF568 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20102-1	CF568 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20099	CF568 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20103	CF568 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20103-1	CF568 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20096	CF568 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20096-1	CF568 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20091	CF568 Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20091-1	CF568 Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20107	CF568 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20107-1	CF568 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20093	CF568 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20093-1	CF568 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20094	CF568 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20094-1	CF568 Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75

Table B. Other CF568 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29010	Annexin V, CF568 conjugate, 50 ug/mL	0.5 mL	$220
00006	CF568 a-Bungarotoxin	0.5 mg	$300
29035	CF568 Streptavidin	1 mg	$160
00044	Phalloidin, CF568 conjugate	300 U	$305

Table C. Reactive CF568 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92154	CF568 hydrazide	1 mg	$150
92024	CF568 maleimide	1 umole	$205
92215	CF568 SE protein labeling kit	3 labelings	$365
92131	CF568 succinimidyl ester	1 umole	$225
92235	Mix-n-Stain CF568 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92275	Mix-n-Stain CF568 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

CF™555 Dye

CF™555 is an orange-red fluorescent dye spectrally similar to Cy3 and Alexa Fluor®555. Like Alexa Fluor® 555, CF™555 is much brighter and more photostable than Cy®3. However, unlike Alexa Fluor® 555, which carries 4 negative charges, CF™555 achieves its superior performance without being excessively charged. Labeling antibodies with a highly charged dye can dramatically change the isoelectric point of the antibody, leading to increased background staining. Figures 1.x and 1.x compare CF™555, Cy®3 and Alexa Fluor® 555 in brightness and photostability. Available reactive forms of CF™555 dye include succinimidyl ester (SE), maleimide, methanethiosulfonate (MTS), amine and hydrazide. Biotium also provides CF™405M-labeled conjugates of various secondary IgG antibodies, streptavidin, phalloidin and a-bungarotoxin. See individual sections for details on these specific products. See Table A-C for the physical property of CF™ dyes.

Table A. CF555 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20039	CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL	0.5 mL	$180
20039-1	CF555 Donkey Anti-Goat IgG (H+L) (min X Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, and Rat), 2 mg/mL	50 uL	$75
20276	CF555 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20276-1	CF555 Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20037	CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL	0.5 mL	$180
20037-1	CF555 Donkey Anti-Mouse IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep), 2 mg/mL	50 uL	$75
20038	CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL	0.5 mL	$180
20038-1	CF555 Donkey Anti-Rabbit IgG (H+L) (min X Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rat, and Sheep), 2 mg/mL	50 uL	$75
20234	CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20234-1	CF555 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20032	CF555 F(ab')2 fragment of Goat Anti-Mouse IgG (H+L), 2 mg/mL	0.25 mL	$120
20035	CF555 F(ab')2 fragment of Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.25 mL	$120
20034	CF555 Goat Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20034-1	CF555 Goat Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20036	CF555 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20036-1	CF555 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20230	CF555 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20230-1	CF555 Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20030	CF555 Goat Anti-Mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20030-1	CF555 Goat Anti-Mouse IgG (H+L), 2 mg/mL	50 uL	$60
20231	CF555 Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20231-1	CF555 Goat Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20247	CF555 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20257	CF555 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20267	CF555 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20033	CF555 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20033-1	CF555 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20232	CF555 Goat Anti-Rabbit IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20232-1	CF555 Goat Anti-Rabbit IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20233	CF555 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20233-1	CF555 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20236	CF555 Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20236-1	CF555 Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20235	CF555 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20235-1	CF555 Rabbit Anti-Mouse IgG (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20031	CF555 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20031-1	CF555 Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60

Table B. Other CF555 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29004	Annexin V, CF555 conjugate, 50 ug/mL	0.5 mL	$220
00018	CF555 a-Bungarotoxin	0.5 mg	$315
00040	Phalloidin, CF555 conjugate	300 U	$305

Table C. Reactive CF555 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92214	CF555 SE protein labeling kit	3 labelings	$365
92130	CF555 succinimidyl ester	1 umole	$225
92254	Mix-n-Stain CF555 antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92234	Mix-n-Stain CF555 antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92274	Mix-n-Stain CF555 antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™488A Dye
A superior green dye to Alexa Fluor 488

CF™488A is a green fluorescent dye optimally excited by the 488 nm argon laser line. Under common detection conditions, CF™488A is at least as bright as Alexa Fluor^® 488. However, a major advantage of CF™488A over Alexa Fluor^® 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor^® 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor^® 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF™488A offers the advantage of less fluorescence “spill-over” in the red channel in multi-color detection applications.

A list of CF™488A-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes. Please visit the Biotium website at www.biotium.com for details.

Technical Summary Abs/Em Maxima: 490/515 nm Extinction coefficient: 70,000 Molecular weight: ~910 Flow cytometry laser line: 488 nm Microscopy laser line: 488 nm Direct replacement for: Alexa Fluor® 488, DyLight™488, FITC, FAM, Cy™2 Advantages Yields biologically more specific antibody conjugates and has less “spill-over” fluorescence in the red channel than Alexa Fluor® 488 Extremely photostable Highly water-soluble and pH-insensitive

Figure 1. Absorption and emission spectra of CF™488A (blue), Alexa Fluor® 488 (green) and FITC (red) conjugated to goat anti-mouse IgG in PBS.

Figure 2. Jurkat cells were stained with 0.5 ug intracellular CD3 or isotype control (BD Biosciences) followed by 1 ug goat anti-mouse IgG conjugates from the manufacturer’s shown above. Fluorescence was analyzed on a Beckman Coulter FC-500. The bars represent the median values of the gated population of cells. The histogram depicts CF488A (green), AlexaFluor488 (blue), DyLight488 Pierce (purple) and DyLight488 KPL (red) stained cells.		Figure 3. Cryosections (6 µm) of human control heart sections stained with anti-fibronectin followed by goat anti-rabbit IgG labeled with CF™488A, Cy™2 and Alexa Fluor® 488, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim (Hessen)-Germany

Table A. CF488A Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20289	Bovine serum albumin, CF488A conjugate	5x1mg	$170
20293	CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20293-1	CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20225	CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL	0.5 mL	$150
20225-1	CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL	50 uL	$65
20208	CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL	0.5 mL	$150
20208-1	CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL	50 uL	$60
20209	CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL	0.5 mL	$150
20209-1	CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL	50 uL	$60
20166	CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20166-1	CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50 uL	$75
20016	CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20016-1	CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20169	CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20169-1	CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20074	CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20074-1	CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20014	CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20014-1	CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20015	CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20015-1	CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20027	CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20027-1	CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20024	CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20024-1	CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20020	CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20020-1	CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20017	CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20017-1	CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20022	CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20022-1	CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20010	CF488A Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20010-1	CF488A Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20011	CF488A Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20302	CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	0.5 mL	$235
20302-1	CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL	50 uL	$100
20018	CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20018-1	CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20246	CF488A Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25mL	$195
20256	CF488A Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25mL	$195
20256-1	CF488A Goat anti-mouse IgG2a, (γ2a), 2mg/mL	50 uL	$85
20266	CF488A Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25mL	$195
20012	CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20012-1	CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20013	CF488A Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20019	CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20019-1	CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20023	CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20023-1	CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20028	CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL	0.5 mL	$150
20028-1	CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL	50 uL	$60
20228	CF488A Monoclonal Mouse Anti-6X His IgG, 1mg/mL	50 uL	$285
20204	CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL	0.25 mL	$180
20204-1	CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL	50 uL	$85
20210	CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL	0.25 mL	$195
20210-1	CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL	50 uL	$95
20215	CF488A monoclonal mouse anti-GFP IgG, 1 mg/mL	0.1 mL	$220
20079	CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	0.5 mL	$150
20079-1	CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL	50 uL	$60
20021	CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL	0.5 mL	$150
20021-1	CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL	50 uL	$60
20071	CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20071-1	CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20026	CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20026-1	CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20025	CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20025-1	CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20172	CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20172-1	CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75

Table B. Other CF488A dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29005	Annexin V, CF488A conjugate, 50 ug/mL	0.5 mL	$220
00005	CF488A a-Bungarotoxin	0.5 mg	$300
29016	CF488A Concanavalin A (Con A)	5x1 mg	$130
29034	CF488A Streptavidin	1 mg	$160
00042	Phalloidin, CF488A conjugate	300 U	$305
29022	Wheat Germ Agglutinin, CF488A conjugate	5x1mg	$250
29022-1	Wheat Germ Agglutinin, CF488A conjugate	1mg	$60

Table C. Reactive CF488A Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92152	CF488A hydrazide	1 mg	$150
92022	CF488A maleimide	1 umole	$205
92213	CF488A SE protein labeling kit	3 labelings	$365
92120	CF488A succinimidyl ester	1 umole	$225
92051	CF488A, aminooxy	1 mg	$390
92253	Mix-n-Stain CF488A antibody labeling kit, 1x(20-50 ug) labeling	1 labeling	$105
92233	Mix-n-Stain CF488A antibody labeling kit, 1x(50-100 ug) labeling	1 labeling	$115
92273	Mix-n-Stain CF488A antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™485 Dye

CF™485M is an unique green fluorescent dye with absorption and emission wavelengths significantly shorter than those of other commonly known green dyes. CF™485 can be efficiently excited by the 470 nm blue LED light or the 488 nm argon laser. The dye’s emission peak wavelength at 513 nm is more than 10 nm shorter than that of FITC or fluorescein, resulting in minimal fluorescence in the red detection channel in flow cytometry or PCR application. Secondary antibody conjugates prepared from the dye are available (See Tables A ). We do not recommend thios dye for microscopy application. See Table A-C for the physical property of CF™ dyes.

Table A. CF485 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20150	CF485 goat anti-mouse IgG(H+L), 2 mg/mL	0.5 mL	$150
20150-1	CF485 goat anti-mouse IgG(H+L), 2 mg/mL	50 uL	$60
20151	CF485 goat anti-rabbit IgG(H+L), 2 mg/mL	0.5 mL	$150
20151-1	CF485 goat anti-rabbit IgG(H+L), 2 mg/mL	50 uL	$60

Table B. Other CF485 dye-labeled Products

Cat#

Product Name

Unit Size

Unit Price

Table C. Reactive CF485 Dyes and Labeling Kits

Cat#

Product Name

Unit Size

Unit Price

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™405M Dye
A superior 405 nm-excitable blue fluorescent dye

CF™405M is a blue fluorescent dye spectrally similar to Pacific Blue® dye (Figure 1). CF™405M is as bright as Pacific Blue® dye but is significantly more photostable than the latter (Figures 2 and 4). An additional advantage of CF™405M is that it has less spillover in the 525/50 green channel than Pacific Blue® dye due to narrower emission peak (Figure 1), making CF™405M a superior choice in multi-color detection applications. We also offer CF™405S, another 405 nm-excitable dye with an even shorter emission wavelength (See CF™405S Flyer for details).
A list of CF™405M-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 408/452 nm Extinction coefficient: 41,000 Molecular weight: 503 Flow cytometry laser line: 405 nm Microscopy laser line: 405 nm Direct replacement for: Pacific Blue® dye, BD Horizon™ V450 Advantages More photostable than Pacific Blue® dye As bright as Pacific Blue® dye in the 450/50 blue channel while having less spillover in the 525/50 green channel Highly water-soluble
		Figure 1. Absorption and emission spectra of CF™405M and Pacific Blue® dye conjugated to goat anti-mouse IgG in PBS.
Figure 2. Jurkat cells were stained with intracellular CD3 followed by CF405M or Pacific Blue goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD LSR II flow cytometer equipped with a 405 violet laser and 450/50 nm emission detection. The bars represent the relative fluorescence of the geometric means of the population of cells. Background was determined by secondary antibody staining alone.		Figure 3. Photostability of CF405M and Pacific Blue dyes. CF405M and Pacific Blue dye solutions at the same concentration were continuously exposed to a mercury arc lamp microscope with a DAPI filter set. Images were taken at every 5 seconds. Fluorescence intensity was normalized to t = 0.

 

Table A. CF405M Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20180	CF405M Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20180-1	CF405M Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20182	CF405M Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20182-1	CF405M Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20181	CF405M Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20181-1	CF405M Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60

Table B. Other CF405M dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29009	Annexin V, CF405M conjugate, 50 ug/mL	0.5 mL	$220
29033	CF405M Streptavidin	1 mg	$160

Table C. Reactive CF405M Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92021	CF405M maleimide	1 umole	$205
92212	CF405M SE protein labeling kit	3 labelings	$365
92111	CF405M succinimidyl ester	1 umole	$225
92252	Mix-n-Stain CF405M Antibody Labeling Kit, 1X(20-50ug) labeling	1 labeling	$105
92232	Mix-n-Stain CF405M Antibody Labeling Kit, 1X(50-100ug) labeling	1 labeling	$115
92272	Mix-n-Stain CF405M antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™405S Dye
Outshining Alexa Fluor® 405!

CF™405S is a blue fluorescent dye with an absorption peak wavelength that nearly coincides with the 405 nm blue diode laser line (Figure 1). In addition, the emission peak wavelength of the dye well centers within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF™405S dye results in much brighter signal than that using Alexa Fluor™ 405 (Figure 2).

A list of CF™405S-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 404/431 nm Extinction coefficient: 33,000 Molecular weight: 1,169 Flow cytometry laser line: 405 nm Microscopy laser line: 405 nm Direct replacement for: Alexa Fluor® 405, Cascade Blue® dye, DyLight™ 405 Advantages Much brighter than Alexa Fluor® 405 due to better compatibility with both excitation and emission windows on common instruments Highly water-soluble pH-insensitive
	Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD LSRII (405 laser, Em 450/50 nm). The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 1. Absorption and emission spectra of CF™405S and Alexa Fluor® 405 conjugated to goat anti-mouse IgG in PBS. The shaded area represents the wavelength window of the 450/50 filter on a BD LSR II flow cytometer.	Figure 3. HeLa cells stained with mouse-Golgin 97 and rabbit COX IV followed by goat anti-mouse CF405S (blue Golgi), goat anti-rabbit CF488A (green mitochondria) and CF555 phalloidin (red F-actin).

 

Table A. CF405S Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20080	CF405S Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20080-1	CF405S Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20082	CF405S Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20082-1	CF405S Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20203	CF405S Monoclonal Mouse Anti-Biotin, 2 mg/mL	0.25 mL	$180
20203-1	CF405S Monoclonal Mouse Anti-Biotin, 2 mg/mL	50 uL	$85

Table B. Other CF405S dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
00002	CF405S a-Bungarotoxin	0.5mg	$315
29032	CF405S Streptavidin	1 mg	$160
29027	Wheat Germ Agglutinin, CF405S conjugate	5X1mg	$265
29027-1	Wheat Germ Agglutinin, CF405S conjugate	1 mg	$65

Table C. Reactive CF405S Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92030	CF405S maleimide	1 umole	$205
92211	CF405S SE protein labeling kit	3 labelings	$365
92110	CF405S succinimidyl ester	1 umole	$225
92251	Mix-n-Stain CF405S antibody labeling kit, 1X(20-50ug) labeling	1 labeling	$105
92231	Mix-n-Stain CF405S antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115
92271	Mix-n-Stain CF405S antibody labeling kit, 1X(5-20ug) labeling	1 labeling	$95

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.

 

CF™350 Dye
A superior UV-excitable blue fluorescent dye

CF™350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, CF™350 is more water-soluble, more photostable and at least 50% more fluorescent on proteins. CF™350 is at least as bright as Alexa fluor® 350 and thus can be a direct replacement for the latter for all applications (Figure 2).

A list of CF™350-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.

Technical Summary Abs/Em Maxima: 347/448 nm Extinction coefficient: 18,000 Molecular weight: 496 Flow cytometry laser line: UV Microscopy laser line: UV nm Direct replacement for: Alexa Fluor® 350, AMCA Advantages Yields the brightest blue fluorescent antibody conjugates when excited at ~350 nm Highly water-soluble Insensitive to pH
	Figure 1. Absorption and emission spectra of CF™350 conjugated to goat anti-mouse IgG in PBS.
	Figure 3. HeLa cells were fixed, permeabilized and stained with mouse anti-alpha-tubulin followed by CF350 goat anti-mouse IgG. Image was taken on an Olympus mercury arc lamp microscope.
Figure 2. Relative fluorescence of CF™350- and Alexa Fluor® 350-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 350 nm excitation.

 

Table A. CF350 Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	Unit Size	Unit Price
20275	CF350 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	0.5 mL	$180
20275-1	CF350 Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL	50uL	$85
20142	CF350 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20142-1	CF350 Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20350	CF350 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20350-1	CF350 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20351	CF350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20351-1	CF350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20148	CF350 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	0.5 mL	$180
20148-1	CF350 Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL	50 uL	$75
20198	CF350 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	0.5 mL	$150
20198-1	CF350 Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL	50 uL	$60
20140	CF350 Goat anti-mouse IgG (H+L), 2 mg/mL	0.5 mL	$150
20140-1	CF350 Goat anti-mouse IgG (H+L), 2 mg/mL	50 uL	$60
20145	CF350 Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20143	CF350 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20143-1	CF350 Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20245	CF350 Goat anti-mouse IgG1, (γ1), 2 mg/mL	0.25 mL	$195
20255	CF350 Goat anti-mouse IgG2a, (γ2a), 2mg/mL	0.25 mL	$195
20265	CF350 Goat anti-mouse IgG2b, (γ2b), 2mg/mL	0.25 mL	$195
20141	CF350 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	0.5 mL	$150
20141-1	CF350 Goat Anti-Rabbit IgG (H+L), 2 mg/mL	50 uL	$60
20146	CF350 Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL	0.25 mL	$120
20144	CF350 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20144-1	CF350 Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20147	CF350 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20147-1	CF350 Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75
20149	CF350 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	0.5 mL	$180
20149-1	CF350 Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL	50 uL	$75

Table B. Other CF350 dye-labeled Products

Cat#	Product Name	Unit Size	Unit Price
29012	Annexin V, CF350 conjugate, 50 ug/mL	0.5 mL	$220
29015	CF350 Concanavalin A (Con A)	5x1 mg	$130
29031	CF350 Streptavidin	1 mg	$160
00049	Phalloidin, CF350 conjugate	300 U	$305
29021	Wheat Germ Agglutinin, CF350 conjugate	5x1mg	$250
29021-1	Wheat Germ Agglutinin, CF350 conjugate	1mg	$60

Table C. Reactive CF350 Dyes and Labeling Kits

Cat#	Product Name	Unit Size	Unit Price
92151	CF350 hydrazide	1 mg	$150
92020	CF350 maleimide	1 umole	$205
92210	CF350 SE protein labeling kit	3 labelings	$365
92109	CF350 succinimidyl ester	1 umole	$225
92050	CF350, aminooxy	1 mg	$390
92230	Mix-n-Stain CF350 antibody labeling kit, 1X(50-100ug) labeling	1 labeling	$115

**Price is for US enduser only. International price may vary. Please contact your local distributors for your price.