CF™488A Dye
A superior green dye to Alexa Fluor 488
CF™488A is a green fluorescent dye optimally excited by the 488 nm argon laser line. Under common detection conditions, CF™488A is at least as bright as Alexa Fluor® 488. However, a major advantage of CF™488A over Alexa Fluor® 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor® 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor® 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF™488A offers the advantage of less fluorescence “spill-over” in the red channel in multi-color detection applications.
A list of CF™488A-based products are shown in Table A-C. A full selection of secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, annexin V and a-bungarotoxin are also available for many CF™ dyes.
Technical Summary
Advantages
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Figure 1. Absorption and emission spectra of CF™488A (blue), Alexa Fluor® 488 (green) and FITC (red) conjugated to goat anti-mouse IgG in PBS. |
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| Figure 2. Jurkat cells were stained with 0.5 ug intracellular CD3 or isotype control (BD Biosciences) followed by 1 ug goat anti-mouse IgG conjugates from the manufacturer’s shown above. Fluorescence was analyzed on a Beckman Coulter FC-500. The bars represent the median values of the gated population of cells. The histogram depicts CF488A (green), AlexaFluor488 (blue), DyLight488 Pierce (purple) and DyLight488 KPL (red) stained cells. | Figure 3. Cryosections (6 µm) of human control heart sections stained with anti-fibronectin followed by goat anti-rabbit IgG labeled with CF™488A, Cy™2 and Alexa Fluor® 488, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim (Hessen)-Germany |
Table A. CF488A Dye-labeled Secondary Antibody Conjugates
Cat# |
Product Name |
Unit Size |
Unit Price |
| Bovine serum albumin, CF488A conjugate | 5x1mg |
$170 |
|
| CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 0.5 mL |
$150 |
|
| CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 50 uL |
$65 |
|
| CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 0.5 mL |
$150 |
|
| CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 50 uL |
$60 |
|
| CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 0.5 mL |
$150 |
|
| CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 50 uL |
$60 |
|
| CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
|
| CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 0.5 mL |
$150 |
|
| CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 50 uL |
$60 |
|
| CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 0.5 mL |
$150 |
|
| CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 50 uL |
$60 |
|
| CF488A Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL |
$120 |
|
| CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 0.5 mL |
$235 |
|
| CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 50 uL |
$100 |
|
| CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Goat anti-mouse IgG1, (γ1), 2 mg/mL | 0.25mL |
$195 |
|
| CF488A Goat anti-mouse IgG2a, (γ2a), 2mg/mL | 0.25mL |
$195 |
|
| CF488A Goat anti-mouse IgG2a, (γ2a), 2mg/mL | 50 uL |
$85 |
|
| CF488A Goat anti-mouse IgG2b, (γ2b), 2mg/mL | 0.25mL |
$195 |
|
| CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 0.5 mL |
$150 |
|
| CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 50 uL |
$60 |
|
| CF488A Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL |
$120 |
|
| CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 0.5 mL |
$150 |
|
| CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 50 uL |
$60 |
|
| CF488A Monoclonal Mouse Anti-6X His IgG, 1mg/mL | 50 uL |
$285 |
|
| CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 0.25 mL |
$180 |
|
| CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 50 uL |
$85 |
|
| CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 0.25 mL |
$195 |
|
| CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 50 uL |
$95 |
|
| CF488A monoclonal mouse anti-GFP IgG, 1 mg/mL | 0.1 mL |
$220 |
|
| CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 0.5 mL |
$150 |
|
| CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 50 uL |
$60 |
|
| CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 0.5 mL |
$150 |
|
| CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 50 uL |
$60 |
|
| CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL |
$180 |
|
| CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL |
$75 |
|
| CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL |
$180 |
|
| CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL |
$75 |
Table B. Other CF488A dye-labeled Products
Cat# |
Product Name |
Unit Size |
Unit Price |
| Annexin V, CF488A conjugate, 50 ug/mL | 0.5 mL |
$220 |
|
| CF488A a-Bungarotoxin | 0.5 mg |
$300 |
|
| CF488A Concanavalin A (Con A) | 5x1 mg |
$130 |
|
| CF488A Streptavidin | 1 mg |
$160 |
|
| Phalloidin, CF488A conjugate | 300 U |
$305 |
|
| Wheat Germ Agglutinin, CF488A conjugate | 5x1mg |
$250 |
|
| Wheat Germ Agglutinin, CF488A conjugate | 1mg |
$60 |
Table C. Reactive CF488A Dyes and Labeling Kits
Cat# |
Product Name |
Unit Size |
Unit Price |
| CF488A hydrazide | 1 mg |
$150 |
|
| CF488A maleimide | 1 umole |
$205 |
|
| CF488A SE protein labeling kit | 3 labelings |
$365 |
|
| CF488A succinimidyl ester | 1 umole |
$225 |
|
| CF488A, aminooxy | 1 mg |
$390 |
|
| Mix-n-Stain CF488A antibody labeling kit, 1x(20-50 ug) labeling | 1 labeling |
$105 |
|
| Mix-n-Stain CF488A antibody labeling kit, 1x(50-100 ug) labeling | 1 labeling |
$115 |
|
| Mix-n-Stain CF488A antibody labeling kit, 1X(5-20ug) labeling | 1 labeling |
$95 |
| **Price is for US enduser only. International price may vary. Please contact your local distributors for your price. |