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AccuBlue™

High Sensitivity and Broad Range dsDNA Quantitation

Superior sensitivity and broad dynamic range

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AccuBlue™ dsDNA quantitation kits offer ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having a similar detection limit but with more convenience and better safety. PicoGreen™ and the high sensitivity Quant-iT™ DNA-binding dye readily crosses cell membranes, whereas the AccuBlue high sensitivity reagent is impermeable to cell membranes, decreasing its potential mutagenicity (Fig. 7). Moreover, the AccuBlue™ reagent is extremely stable. Supplied with a 1X aqueous quantitation solution, enhancer, and DNA standards, AccuBlue™ can be conveniently stored at 4oC, unlike PicoGreen™ or the Quant-iT™ reagents, which have to be aliquoted for storage to avoid freeze-thaws. The kits are compatible with microplate readers, spectrofluorometers and hand-held minifluorometers such as the Qubit™ from Invitrogen. The kits provide sufficient reagents to perform 1000 assays based on a 96-well microplate format.

* AccuBlue is a trademark of Biotium, Inc.; PicoGreen, Qubit and Quant-iT are trademarks of Invitrogen Corp.
** AccuBlue is covered by pending domestic and international patents.

Figure 1: Triplicate samples of calf thymus dsDNA, mouse liver RNA or M13mp18 viral ssDNA were assayed with the AccuBlue High Sensitivity dsDNA Quantitation Kit. Fluorescence was measured at 485/530 nm and plotted vs. the mass of the nucleic acids.		Figure 2: AccuBlue can quantify a large number of dsDNA samples in four easy steps.

Figure 3: Two-fold dilutions of calf thymus DNA were assayed using the AccuBlue HS kit with the QubitTM fluorometer. Inset shows the lower end of the titration.		Figure 4: Two-fold dilutions of calf thymus DNA were assayed using AccuBlue BR on a microplate reader. Inset shows the lower end of the titration.
Figure 5: AccuBlue High Sensitivity Reagent and PicoGreen stability was measured by reading absorbance of reagents in aqueous buffer at 5 min intervals at room temperature. The PicoGreen reagent decomposes quickly.		Figure 6: Two-fold dilutions of calf thymus DNA were assayed using AccuBlue or Quant-iT Broad Range assay kits. AccuBlue has improved linearity and wider dynamic range than the Quant-iT Broad Range.

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